human spp1 quantikine elisa (R&D Systems)

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R&D Systems human spp1 quantikine elisa

(A) Sankey plot showing top 30 differential LR interactions from OsteoCAR cells through ECM glycoproteins towards other co-localized cells. Colors represent LR expression per interaction: orange indicates stronger signaling in disease, blue indicates weaker signaling in disease. (B) Volcano plot for differential gene expression of OsteoCAR-Fibroblast multiplets cell. Genes with logFC > 1 and p-value < 0.05 shown in red; genes with p-value < 0.05 but low logFC in blue and others in green. (C) Representative images of <t>Spp1</t> staining and quantification of mean DAB intensity in 4um femoral sections of TPO– or EV-induced murine BM fibrosis. Scale bar: 50um. P-value is based on an unpaired t-test.(*) = p<0.05. (D) Schematic representation of experimental timeline, where WT ckit+ donor cells are transduced with TPO-GFP or EV-GFP control lentiviral vectors, and transplanted into lethally-irradiated Spp1KO recipient mice (n=4-5/group). (E) Hemoglobin and red blood cell counts in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. (F) Blood monocytes quantified by flow cytometry in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). (G) Spleen weight in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. (H) Image of spleens isolated from TPO-driven fibrosis in WT and Spp1KO recipient mice at sacrifice. (I) Bone marrow cellularity in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). (J) Quantification of reticulin staining in 4um femoral sections in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). (K) Representative imaging of reticulin staining in TPO-driven fibrosis in WT and Spp1KO recipient mice. Scale bar: 50um.

Human Spp1 Quantikine Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 160 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+osteopontin/Human+Osteopontin+(OPN)+Quantikine+ELISA+Kit/bio_rxiv__64898__2026__03__16__711605-307-11-15
Average 95 stars, based on 160 article reviews

human spp1 quantikine elisa - by Bioz Stars, 2026-09

95/100 stars

Images

1) Product Images from "NicheSphere reveals Spp1⁺ macrophages as central hubs coordinating fibrotic remodeling in myeloproliferative neoplasms"

Article Title: NicheSphere reveals Spp1⁺ macrophages as central hubs coordinating fibrotic remodeling in myeloproliferative neoplasms

Journal: bioRxiv

doi: 10.64898/2026.03.16.711605

Figure Legend Snippet: (A) Sankey plot showing top 30 differential LR interactions from OsteoCAR cells through ECM glycoproteins towards other co-localized cells. Colors represent LR expression per interaction: orange indicates stronger signaling in disease, blue indicates weaker signaling in disease. (B) Volcano plot for differential gene expression of OsteoCAR-Fibroblast multiplets cell. Genes with logFC > 1 and p-value < 0.05 shown in red; genes with p-value < 0.05 but low logFC in blue and others in green. (C) Representative images of Spp1 staining and quantification of mean DAB intensity in 4um femoral sections of TPO– or EV-induced murine BM fibrosis. Scale bar: 50um. P-value is based on an unpaired t-test.(*) = p<0.05. (D) Schematic representation of experimental timeline, where WT ckit+ donor cells are transduced with TPO-GFP or EV-GFP control lentiviral vectors, and transplanted into lethally-irradiated Spp1KO recipient mice (n=4-5/group). (E) Hemoglobin and red blood cell counts in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. (F) Blood monocytes quantified by flow cytometry in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). (G) Spleen weight in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. (H) Image of spleens isolated from TPO-driven fibrosis in WT and Spp1KO recipient mice at sacrifice. (I) Bone marrow cellularity in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). (J) Quantification of reticulin staining in 4um femoral sections in TPO-driven fibrosis in WT and Spp1KO recipient mice at the time of sacrifice (82 days post-transplant). (K) Representative imaging of reticulin staining in TPO-driven fibrosis in WT and Spp1KO recipient mice. Scale bar: 50um.

Techniques Used: Expressing, Gene Expression, Staining, Transduction, Control, Irradiation, Flow Cytometry, Isolation, Imaging

(A) Sankey plot showing top 25 LR pairs associated with Spp1+ macrophage-driven signaling via Spp1 as a ligand. (B) Heatmap of pathway score based on SPP1-associated intracellular signaling, highlighting differential transcriptional response between TPO vs. EV. Higher scores indicate stronger evidence of differential pathway regulation due to up-stream SPP1 signaling. (C) Sankey plot showing SPP1-associated intracellular signaling in Ly6C low infiltrating macrophages and resident-like macrophages, and pathways with enriched differential pathway score from panel B. Genes on the right represent genes downstream of SPP1. Here, only target genes that are ligands are shown. The color map reflects the log-transformed fold change (logFC) between TPO vs. EV. (D) Cytokines localized differential communication network showing cell types (nodes) colored by Niche with node size based on betweenness score. Edge colors represent normalized differential communication scores for cell type pairs. Red color in edges indicates increased signaling in disease, blue indicates decreased signaling in disease. (E) mRNA expression of Col1a1, αSMA and Spp1 in Gli1tdtomato MSCs stimulated with DMSO control, Tgf-β, IL-6, IL-1a, IL-1b and S100A8 for 48 hours, n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. (F) mRNA expression of Col1a1, Spp1 and IL-6 in Gli1tdtomato MSCs stimulated with DMSO control, Tgf-β and recombinant Spp1 for 48 hours, n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001.

Figure Legend Snippet: (A) Sankey plot showing top 25 LR pairs associated with Spp1+ macrophage-driven signaling via Spp1 as a ligand. (B) Heatmap of pathway score based on SPP1-associated intracellular signaling, highlighting differential transcriptional response between TPO vs. EV. Higher scores indicate stronger evidence of differential pathway regulation due to up-stream SPP1 signaling. (C) Sankey plot showing SPP1-associated intracellular signaling in Ly6C low infiltrating macrophages and resident-like macrophages, and pathways with enriched differential pathway score from panel B. Genes on the right represent genes downstream of SPP1. Here, only target genes that are ligands are shown. The color map reflects the log-transformed fold change (logFC) between TPO vs. EV. (D) Cytokines localized differential communication network showing cell types (nodes) colored by Niche with node size based on betweenness score. Edge colors represent normalized differential communication scores for cell type pairs. Red color in edges indicates increased signaling in disease, blue indicates decreased signaling in disease. (E) mRNA expression of Col1a1, αSMA and Spp1 in Gli1tdtomato MSCs stimulated with DMSO control, Tgf-β, IL-6, IL-1a, IL-1b and S100A8 for 48 hours, n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. (F) mRNA expression of Col1a1, Spp1 and IL-6 in Gli1tdtomato MSCs stimulated with DMSO control, Tgf-β and recombinant Spp1 for 48 hours, n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001.

Techniques Used: Transformation Assay, Expressing, Control, Recombinant

A. Schematic representation of experimental timeline, where WT or Spp1KO ckit+ donor cells are transduced with TPO-GFP or EV-GFP control lentiviral vectors, and transplanted into lethally-irradiated WT recipient mice (n=5/group, experiment duration: 55 days after transplant). B. Hemoglobin and red blood cell counts in TPO-driven fibrosis in WT recipient mice with WT or Spp1KO BM at the time of sacrifice (55 days post-transplant). P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. C. Blood CD11b+ F4/80+ monocyte-derived macrophages quantified by flow cytometry in TPO-driven WT or Spp1KO fibrosis in WT recipient mice at the time of sacrifice (55 days post-transplant). D. Quantification of reticulin staining in 4um femoral sections in EV– or TPO-driven fibrosis in WT or Spp1KO-driven fibrosis at the time of sacrifice (55 days post-transplant). E. Representative imaging of reticulin staining in 4um femoral sections in TPO-driven WT or Spp1KO-driven fibrosis in WT recipient mice. Scale bar: 50um. F. ELISA analysis of IL-1b protein in plasma of WT or Spp1KO-driven TPO fibrosis. n=3-5/group. G. Representative imaging of IL-1b staining and DAB quantification in 4um femoral sections TPO-driven WT or Spp1KO-driven fibrosis in WT recipient mice. Scale bar: 50um. H. mRNA expression of Spp1, Tnfa and Il-6 in WT or Spp1KO FL-HoxB8 cells (undifferentiated) carrying either the EV or MPLW515L mutation. n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. I. Flow cytometry analysis of macrophage differentiation (CD11b+ F4/80+ cells) from FL-HoxB8 cells (WT vs. Spp1KO, MPLW515L vs. EV) over 5 days, followed by PE+ latex bead phagocytosis assay performed for 1 hour. n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05, (****) = p<0.0001.

Figure Legend Snippet: A. Schematic representation of experimental timeline, where WT or Spp1KO ckit+ donor cells are transduced with TPO-GFP or EV-GFP control lentiviral vectors, and transplanted into lethally-irradiated WT recipient mice (n=5/group, experiment duration: 55 days after transplant). B. Hemoglobin and red blood cell counts in TPO-driven fibrosis in WT recipient mice with WT or Spp1KO BM at the time of sacrifice (55 days post-transplant). P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. C. Blood CD11b+ F4/80+ monocyte-derived macrophages quantified by flow cytometry in TPO-driven WT or Spp1KO fibrosis in WT recipient mice at the time of sacrifice (55 days post-transplant). D. Quantification of reticulin staining in 4um femoral sections in EV– or TPO-driven fibrosis in WT or Spp1KO-driven fibrosis at the time of sacrifice (55 days post-transplant). E. Representative imaging of reticulin staining in 4um femoral sections in TPO-driven WT or Spp1KO-driven fibrosis in WT recipient mice. Scale bar: 50um. F. ELISA analysis of IL-1b protein in plasma of WT or Spp1KO-driven TPO fibrosis. n=3-5/group. G. Representative imaging of IL-1b staining and DAB quantification in 4um femoral sections TPO-driven WT or Spp1KO-driven fibrosis in WT recipient mice. Scale bar: 50um. H. mRNA expression of Spp1, Tnfa and Il-6 in WT or Spp1KO FL-HoxB8 cells (undifferentiated) carrying either the EV or MPLW515L mutation. n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05,(**)= p<0.01, (***) = p<0.001, (****) = p<0.0001. I. Flow cytometry analysis of macrophage differentiation (CD11b+ F4/80+ cells) from FL-HoxB8 cells (WT vs. Spp1KO, MPLW515L vs. EV) over 5 days, followed by PE+ latex bead phagocytosis assay performed for 1 hour. n=3/group, P-value is based on a one-way ANOVA.(*) = p<0.05, (****) = p<0.0001.

Techniques Used: Transduction, Control, Irradiation, Derivative Assay, Flow Cytometry, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Expressing, Mutagenesis, Phagocytosis Assay

(A) ELISA analysis of SPP1 protein in human blood plasma of MPN patients and healthy controls. n=6 healthy, 15 MPN. Statistical test performed is an unpaired t-test, p=0.2796. (B) Normalized SPP1 plasma protein abundance in MPN patients for distinct outcomes. Differences between groups were evaluated using a student t-test. (C) Kaplan–Meier survival curves of primary myelofibrosis patients stratified by quartiles of SPP1 concentration at baseline assessment. Differences between groups were evaluated using the log-rank test.

Figure Legend Snippet: (A) ELISA analysis of SPP1 protein in human blood plasma of MPN patients and healthy controls. n=6 healthy, 15 MPN. Statistical test performed is an unpaired t-test, p=0.2796. (B) Normalized SPP1 plasma protein abundance in MPN patients for distinct outcomes. Differences between groups were evaluated using a student t-test. (C) Kaplan–Meier survival curves of primary myelofibrosis patients stratified by quartiles of SPP1 concentration at baseline assessment. Differences between groups were evaluated using the log-rank test.

Techniques Used: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Quantitative Proteomics, Concentration Assay

Figure Legend Snippet:

Techniques Used:

Recombinant:

Article Title: Integrated histopathology, spatial and single cell transcriptomics resolve cellular drivers of early and late alveolar damage in COVID-19
Article Snippet: .. Human umbilical vein endothelial cells (HUVECs; Lonza, C2519A) were treated with recombinant human osteopontin (rhOPN; R&D Systems, 1433-OP-050) for 24 hours in serum and supplement-free growth medium (EGM, Lonza, CC-3162). .. Following the incubation, cells were fixed in 10% formalin in PBS, then either blocked and permeabilized in 4% (w/v) bovine serum albumin (BSA, Sigma Aldrich, A3059) and 0.2% (v/v) TritonX-100 (Thermo Fisher, 85111) in PBS (Gibco, 20012027) (PAI-1 staining) or blocked in 1% (w/v) BSA and 5% (v/v) normal goat serum (EDM Millipore, S26-100ML) in PBS (ITGAV, ITGB1, ITGA5 staining) for 30 mins at RT.

Article Title: Extracellular 20S proteasome secreted via microvesicles can degrade poorly folded proteins and inhibit Galectin-3 agglutination activity.
Article Snippet: Laboratory of Pathogen Host Interactions, Université Montpellier, CNRS, Montpellier, France Institut des Biomolécules Max Mousseron, Université Montpellier, CNRS, Montpellier, France The Feinstein Institutes for Medical Research, Manhasset, New York, USA Institut de Pharmacologie et de Biologie Structurale, Université Toulouse, CNRS, UPS, Toulouse, France Centre de Recherche en Biologie cellulaire de Montpellier, Univ.. Montpellier, CNRS, Montpellier, France Service de Dermatologie, CHU Nîmes, Nîmes, France Institut de Recherche en Cancérologie de Montpellier (IRCM), Université Montpellier, Montpellier, France

Article Title: Integrated histopathology, spatial and single cell transcriptomics resolve cellular drivers of early and late alveolar damage in COVID-19.
Article Snippet: .. OPN treatment and immunofluorescent staining of endothelial cells Human umbilical vein endothelial cells (HUVECs; Lonza, C2519A) were treated with recombinant human osteopontin at varying concentrations (0 μg/mL, 0.5 μg/mL, 1 μg/mL) (rhOPN; R&D Systems, 1433-OP050) for 24 hours in serum and supplement-free growth medium (EGM, Lonza, CC-3162). .. Following the incubation, cells were fixed in 10% formalin in PBS, then either blocked andpermeabilized in 4% (w/v) bovine serum albumin (BSA, Sigma Aldrich, A3059) and 0.2% (v/v) TritonX-100 (Thermo Fisher, 85111) in PBS (Gibco, 20012027) (PAI1 staining) or blocked in 1% (w/v) BSA and 5% (v/v) normal goat serum (EDMMillipore, S26-100ML) in PBS (ITGAV, ITGB1, ITGA5 staining) for 30mins at RT.

Article Title: Molecular insights into the early stage of glomerular injury in IgA nephropathy using single-cell RNA sequencing.
Article Snippet: Molecular insights into the early stage of glomerular injury in IgA nephropathy using single-cell RNA sequencing Sonia Zambrano, Liqun He, Toshiki Kano, Ying Sun, Emmanuelle Charrin, Mark Lal, Christer Betsholtz, Yusuke Suzuki and Jaakko Patrakka KI/AZ Integrated Cardio Metabolic Centre, Division of Pathology, Department of Laboratory Medicine, Karolinska Institute at Karolinska University Hospital Huddinge, Stockholm, Sweden; Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neuroinjury, Neuro-Repair and Regeneration in Central Nervous System, Tianjin, China; Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden; Department of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan; Bioscience Renal, Research and Early Development, Cardiovascular, Renal and Metabolism, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden; and Department of Medicine Huddinge, Karolinska Institute, Huddinge, Sweden

Article Title: Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties
Article Snippet: The center line average roughness (Ra) was measured using 3D laser microscopy (VK-9710, Keyence, Osaka, Japan). .. The titanium plates were coated with recombinant human osteopontin (rhOPN; 1433-OP-050, R & D Systems, Minneapolis, MN, USA) or recombinant human laminin-332 (rhLN; ReproCELL, Yokohama, Japan). rhOPN and rhLN were diluted with 0.01 M phosphate-buffered saline (PBS) at concentrations of 2.5 μg/mL and 0.5 μg/mL, respectively, and titanium plates were immersed in one of these solutions for 1 h. After rinsing off the unbound protein with PBS, the titanium plates were treated with 10% normal rabbit serum or normal goat serum (Nichirei Bioscience, Tokyo, Japan) for 10 min to prevent nonspecific adsorption of antibodies, and then the titanium plates were incubated with 5 μg/mL diluted goat anti-human OPN polyclonal antibody (AF1433-SP, R & D Systems, Minneapolis, MN, USA) or 4 μg/mL diluted mouse anti-human laminin-332 monoclonal antibody (P3H9-2, Santa Cruz Biotechnology, Dallas, TX, USA) for 1 h. After rinsing unbound antibodies off with PBS, the plates were treated with 10 μg/mL of fluorescein-conjugated rabbit anti-goat IgG or goat anti-mouse IgG (Invitrogen, Carlsbad, CA, USA) for 30 min. After the reaction, the titanium plates were rinsed with distilled water, and fluorescent intensity was measured using a multimode microplate reader (Infinite F200 Pro, Tecan, Salzburg, Austria). ..

Article Title: Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties.
Article Snippet: The center line average roughness (Ra) was measured using 3D laser microscopy (VK-9710, Keyence, Osaka, Japan). .. The titanium plates were coated with recombinant human osteopontin (rhOPN; 1433-OP-050, R & D Systems, Minneapolis, MN, USA) or recombinant human laminin-332 (rhLN; ReproCELL, Yokohama, Japan). rhOPN and rhLN were diluted with 0.01 M phosphate-buffered saline (PBS) at J. Clin. ..